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Image Search Results
Journal: bioRxiv
Article Title: Scaling Multiplex qPCR Primer Design to 1000-plex using the Degenerate Incomplete Multiplex Primer List Extension (DIMPLE) Algorithm
doi: 10.64898/2026.04.17.719221
Figure Lengend Snippet: Demonstration of DIMPLE multiple qPCR primer design for DNA methylation marker detection. (a) Illustration of the products of bisulfite/APOBEC conversion of DNA. For each DNA locus, up to 4 different sets of primers (8 primers) must be designed to maximize sensitivity to the insert sequence. Alternatively, primer specificity can be used to detect specific methylation states of the primer binding regions. (b) Demonstration of 60-plex qPCR amplification of DNA hypomethylation markers. Here, 120 primers were designed to amplify only the + strands of the converted unmethylated DNA molecules, because unmethylated DNA has the lower sequence diversity than converted methylated DNA molecules, and is more challenging for traditional PCR primer design algorithms.
Article Snippet: For methylation assays, the
Techniques: DNA Methylation Assay, Marker, Sequencing, Methylation, Binding Assay, Amplification